cluster of differentiation 68 cd68  (Bio-Rad)

Journal: Particle and Fibre Toxicology

Article Title: Fiber length and shape-dependent differences in hepatic nanomaterial localization in mice following pulmonary exposure

doi: 10.1186/s12989-025-00652-7

Figure Lengend Snippet: Enhanced dark-field microscopy (DFM) and corresponding bright-field images of the same immunohistochemically (IHC) stained liver sections using purple chromogen. (a) Example of fiber (Mitsui-7) localization in hepatocytes. Right: DFM; left: bright-field of the same section stained for the hepatocyte nuclear marker HNF4α. (b) Example of fiber localization in Kupffer cells. Right: DFM; left: bright-field of the same section stained for the macrophage cytoplasmic marker CD68. (c) Example of fibers categorized as “other placement.” Right: DFM; left: bright-field of the same section stained for CD68 and HNF4α. Arrows point to the nanomaterial locations

Article Snippet: Cluster of Differentiation 68 (CD68) (cat. no. 977786, Cell Signaling Technology, Massachusetts, USA) was used as a specific marker for macrophages, and Hepatocyte nuclear factor 4 alpha (HNF4α) (cat. no. AB199431 , Abcam, Cambridge, UK) was used as a marker for hepatocytes [ , ].

Techniques: Microscopy, Staining, Marker

Journal: Nature Communications

Article Title: Fibroblast growth factor signaling induces a chondrocyte-like state of peripheral nerve fibroblast during aging

doi: 10.1038/s41467-025-65297-8

Figure Lengend Snippet: a , b UMAP embedding of 1180 macrophage nuclei from sciatic nerves color-coded by subtype ( a ) or age ( b ). c Dot plot highlighting the marker genes used to identify the macrophage subtypes from A. Marker genes can be found in Table . d Relative percentage of Mϕs subtypes across ages. Steady state epi- and endoneurial Mϕ, circulating-derived phagocytic Mϕ, and chronic-inflammation Mϕ were more prevalent in sciatic nerves from 2-3, 15-16, and 20-30 months old mice, respectively. e Dot plot showing the expression levels of the marker genes used to identify the macrophage clusters, at the different time points. Markers for resident steady state Mϕ ( Cx3cr1, Mrc1, Csf1r ) are more expressed at 2-3 months old, those for circulating-derived phagocytic Mϕ (Cd74, Hspa5, Cd44, C1qa, Grn, Cd68), are more expressed at 15-16 months old, and those belonging to chronic inflammation ( Tgfβr1, Kynu) are more expressed in the 20-30 months old samples. f Representative immunofluorescence images of sciatic nerve samples show increased CD45 + /MRC1 + Mϕs in nerves from 2-3 months old mice, CD68 + /GRN + Mϕs in nerves from 15-16 and 20-30 months old mice, and CD45 + /TGF βR1+ in nerves from 20-30 months old mice. Yellow or pink arrowheads point to CD45 + /MRC1+ or CD68 + /GRN+ or CD45 + / TGF βR1 + cells. g Quantification of the number of CD45+ cells relative to the total number of cells. n = 7 - 9 - 10, biological replicates. h Quantification of the number of CD45 + /MRC1 + cells relative to the total number of CD45 + cells. n = 4 - 4 - 5 biological replicates. i Quantification of the number of CD68 + / GRN+ cells relative to the total number of CD68+ cells. n = 6 - 6 - 5. j Quantification of the number of CD45 + /TGF βR1 + cells relative to the total number of CD45 + cells. n = 3 - 5 - 5, biological replicates. Data are presented as mean values +/− SD. Source data for panels is provided as a Source Data file.

Article Snippet: Cluster of differentiation 68 (CD68) 1:250 , Mouse , MCA1957 Biorad Hercules, CA, USA.

Techniques: Marker, Derivative Assay, Expressing, Immunofluorescence